Carcinogen-specific induction of genetic instability

Induction of genetic instability and chromosomal instability by nickel sulfate in V79 Chinese hamster cells.

Nickel compounds are known to be carcinogenic to humans and show genotoxicity, including the ability to induce chromosome aberrations and neoplastic transformation in vitro. The mutagenicity of nickel compounds is, however, equivocal and the mechanisms of carcinogenesis are still not clear. In this study, the possibility that nickel compounds induce genetic or chromosomal instability was examin...

Site-specific carcinogen binding to DNA.

Benzo[alpha]pyrene diol epoxide (BPDE) is a well-studied environmental carcinogen that binds covalently to DNA. Here we describe a photochemical technique that allows us to map BPDE-binding sites within cloned gene sequences. The technique is based upon our observation that, when irradiated with laser light at 355 nm, one single-strand DNA cut is produced at each BPDE binding site. In initial e...

Tumor induction through varying lengths of carcinogen exposure.

Efficiency Improvement of Induction Motor using Fuzzy-Genetic Algorithm

In most industrial zones, electric energy is one of the most important energy sources. Since electrical motors are the main energy consumers of industrial factories, consumption optimization in these motors can be considered as a main option related to energy saving. One very effective way to reduce the consumption of these equipment is to use a motor speed controllers or drives. Since the loss...

Early induction of genetic instability and apoptosis by arsenic in cultured Chinese hamster cells.

In order to assess at what time from the beginning of exposure inorganic arsenic can give rise to genetic instability and trigger apoptosis, V79-C13 Chinese hamster cells were treated with 10 microM sodium arsenite for 24 h. Under these conditions, cell survival was >70% and cells showed neither an increase in chromosome aberration frequency nor a delay in cell cycle progression. Investigations...